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After centrifugation at 5000 rpm for 10 min, cells were incubated with primary antibodies FSP1 (1:500, Proteintech, 20886-1-AP) and importin 2 (1:200, Santa Cruz, SC-55538) at 4 C overnight

Immunopathology and C4d were reported in the lungs of six fatal cases in this age group, indicating that antibody-dependent complement activation through immune-complex formation may have been a contributing factor