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For the 5-ethynyl-2-deoxyuridine (EdU) assay, approximately 4,000 Huh7 or Hep3B cells were seeded per well in 96-well plates and incubated with 10 M (EdU

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I will link to those so you can take a deeper dive on the whys of HRT And why we're often starting the estrogen progesterone is because that's laying the groundwork for the improved insulin sensitivity