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After incubation, the coverslip was transferred into the original medium for 15 min and for labeling, neurons were three times washed with 1x PBS and fixed with 4 % PFA/ 4 % sucrose in PBS for 15 min at room temperature (RT)

But Coast Guard officials added that the number of prescriptions distributed may not equate to the number of individuals treated, considering there may be multiple prescriptions for the same individual

Digesti n ms lenta : Los alimentos permanecen ms tiempo en el estmago, lo que contribuye a la sensacin de saciedad y a mantener estables los niveles de azcar en sangre