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After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

Naville D, Chatelain PG, Avallet O, Saez JM: Control of production of insulin-like growth factor I by pig Leydig and Sertoli cells cultured alone or together

The second limiting amino acid, representing the next most deficient amino acid, can also negatively impact growth even when the first limiting amino acid is supplemented (58)

SGLT-2 inhibitors and cardiovascular risk in diabetes mellitus: A comprehensive and critical review of the literature