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Before (time point: 0 h) and after incubation for 12, 20 and 40 h at 24 C whilst shaking (150 rpm), aliquots (2.0 mL) were taken, autoclaved (15 min, 121 C), pellets collected by centrifugation (13000 rpm, 4 min) and extracted with EtOH (1 mL) by shaking overnight (15 h) at room temperature

The acceptor GlyGly interacted with five residues (L87, E90, Y305, N327, S348) and perfectly docked in the binding pocket of Y327N

Immunopathol 39 (4), 469486

3.1.3 Hemoglobin Upon release from erythrocytes following hemolysis, hemoglobin exhibits peroxidase-like activity, generating ROS that contribute to its antibacterial function