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Then, the slices were incubated with primary TH antibody (Sigma-Aldrich, T1299) and primary GFAP antibody (Millipore, MAB360) at 4 C overnight and then with the corresponding secondary antibody, and the results were visualized by the DAB reaction

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The microdosing approach Some practitioners use microdosing tirzepatide strategies, where the dose increases occur in smaller increments

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