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Type 2 diabetes is largely preventable and is associated with lifestyle factors such as sedentary behavior, unhealthy diet, excess sugar consumption, and genetic predisposition

During the MD simulations, the temperature was maintained at 300 K using the Langevin damping method, and the pressure was kept at 1 atm using the Langevin piston method

Plasma levels of hepatotoxic makers such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT), as well as albumin concentration, were comparable among tested groups, indicating no significant safety concerns, despite the higher occurrence of knockout over knock-in events (Fig

doi: 10.1016/s0960-0760(99)00030-8