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Viral delivery and evaluation of photoreceptor degeneration in the Rho P23H/+ mouse model P23H/+ The cDNA encoding H105A was cloned in frame with the signal peptide from Interferon beta 38 , to allow secretion of the H105 peptide, in a pAAV2 vector with a CMV promoter resulting in AAV-H105A
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A lower GSH:GSSG ratio results in more oxidizing conditions (240 mV) [70] in the endoplasmic reticulum allowing protein disulfide formation
