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Following euthanization, skin tissues were collected from the shaved area at the nape of the neck onto an ice-cold glass plate at 24 h after the behavioural analysis

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-catenin expression in HCC is correlated with poor prognosis but TH can suppress the Wnt/-catenin pathway by means of Dickkopf-4 induction, leading to inhibition of hepatoma cell proliferation and metastasizing (Figure 4)