CpG Methylase (M. Sssl) Microbial DNA Isolation The DNA from both reactions

The DNA from both reactions is then amplified using Real-Time PCR

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the Quick-DNA/RNA™ Water Kit ensures robust recovery and purification of DNA/RNA

the ZymoPURE – Express Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1

CpG Methylase (M. Sssl) Microbial DNA Isolation The DNA from both reactionsCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.