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This finding highlights the value of untargeted metabolomics in capturing the expected biochemical signature of MMA and suggests that broader metabolite profiling may provide complementary information alongside conventional biochemical testing and confirmatory diagnostic approaches

Together our findings suggest that acetylation is a potent mechanism to regulate TrxR1 activity that also allows the enzyme to evade oxidative inactivation

With these technologies, recombinant Ustilago and related strains may provide powerful tools for enhanced EGT production

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