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The medium-chain acyl-CoA synthetases (ACSMs), including ACSM1, ACSM2A, ACSM2B, ACSM3, and ACSM5, present in the mitochondria, catalyze the formation of fatty acyl-CoA thioesters, i.e., the activated form of MCFAs, using adenosine triphosphate (ATP) and through a thioester linkage between the carboxyl group (OOH) of the MCFAs and the thiol (SH) group of coenzyme A (Co-A) (Watkins 2013

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Timing the preparation is important too