showed that in the absence of DNA, a member of the basic/helix-loop-helix/zipper family of DNA-binding proteins, transcription factor Max, is rapidly digested by several endoproteases, indicating an open and flexible structure of the protein in the unbound form (Cohen et al., 1995)
The situation changed dramatically when it was proposed to use NMR as a method for HDX detection (Dempsey, 2001), and NMR-based HDX detection was the leading technique in the field until the early 2000s, when mass spectrometry (MS) emerged as an extremely powerful alternative (Deng et al., 2016
Activated Akt then phosphorylates eNOS at Ser1177, directly increasing nitric oxide production
Under California law, you exceed the legal limit if you have a BAC of 0.08% or higher