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Methods Bacterial strains and growth conditions Lactococcus lactis subsp

GPX4 presents with a typical thioredoxin motif containing four -helices that are localized near the protein surface, and seven b-strands, five of which cluster together forming a central b-sheet (see Figure 3 for a crystal structure representation of GPX4 adapted from the PDB (6ELW)) [50]

Intracellularly, ROS facilitate the integration of oxidized free polyunsaturated fatty acid products into biological membranes, including plasma and mitochondrial membranes, as shown in Table 1

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