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Gene expression was quantified using SYBR green real-time PCR master mix (TOYOBO, Japan) and amplified under the following conditions: 2 min at 95C, 20 s at 95C, and 20 s at 58C, for 40 cycles

When carbohydrate intake drops below roughly 20 to 50 grams per day, glycogen stores deplete within 24 to 72 hours, and the liver begins converting fatty acids into ketone bodies: beta-hydroxybutyrate, acetoacetate, and acetone

J Am Soc Hypertens 5: 161165