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doi: 10.3389/fmed.2025.1608975 Received 10 April 2025 Accepted 12 June 2025 Published 08 July 2025 Volume 12 - 2025 Edited by Patrice X

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Adding high caffeine and sugar worsens symptoms significantly

To obtain stable SLC25A39 knockdown cells, Huh7 cells were transfected with lentivirus carrying a shRNA targeting SLC25A39 (shRNA-SLC25A39, 5-GTGGTTACCTCTCTCTTCATG-3) or negative control (sh-NC, 5-TTCTCCGAACGTGTCACGT-3)