They were then washed 5 times in PBS and then incubated for 1 h in FITC-conjugated secondary antibody diluted in 2% BSA and 0.2% Triton-X 100 in PBS with DAPI
This figure provides an overview of the key steps, including the creation of mutant mice, breeding, GH treatment, blood sample collection, untargeted metabolomic analysis, data processing, and the subsequent discovery of biomarkers
Lastly, genetic manipulation of antioxidant genes, such as Sod1 , Gpx 1 and Cat , in animal models alters susceptibility to alcohol-induced liver injury 28,29,30
The role of reactive oxygen species in the rheumatoid arthritis-associated synovial microenvironment