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Individual fish were divided using a razor blade into 0.5 cm sections and added to a 40 mL glass tube along with 0.1 mL of DI water (18 M-cm Milli-Q, EMD Millipore, Burlington, MA) whereupon tissues were homogenized into a paste using an Omni TH (Omni Int., Kennesaw, GA) tissue homogenizer