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Sections were then blocked with dual endogenous enzyme-blocking reagent (DEEB, Agilent/Dako, S200389-2) (10 minutes) and Avidin/Biotin/Sniper (Vector, SP-2001) (3 minutes each) in turn, followed by mouse anti-glucagon (Abcam, ab10988) (1:1,000, 30 minutes) and goat anti-mouse AP conjugate (Biocare Medical, MALPH521H) (30 minutes), and detected using Ferangi Blue (Biocare Medical, FB813H) (2 minutes)

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