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For quantification, the islet images were captured systematically covering the whole section in confocal mode on a microscope to evaluate every cluster of insulin-stained cells (three to seven cells) or islets (eight or more cells) per section was evaluated for -H2AX, MOTS-c or IL-1 intensity (A.U.)

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Hypoglycemia: Cases of hypoglycemia have been reported in patients taking opioids